Promega 1kb dna step ladder molecular weight marker for electrophoresis 1kb dna step ladder to 10,000. Hyperladder 1kb with 1 % wv agarose gel, hyperladder 50bp with 1. How can i prepare working solution with 1 kb dna ladder. Promega 1kb dna ladder molecular weight marker 1kb dna ladder. General laboratory supplies can be found in our extensive online collection of products from globally recognized and trusted brands, including promega. General description sigmas directload 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0.
The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Dna marker 100 bp plus is designed for use as qualitative dna reference marker availibility of sample size may be limited. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. This ladder consists of bands containing 1 8 repeats of a 1018bp dna fragment. In this study, we report a new procedure to prepare a dna ladder that consists of 10 fragments from 100 to bp.
The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed. The ladder is dephosphorylated and ready for 5 endlabeling with radioisotopes using t4 polynucleotide kinase, allowing visualization by autoradiography. Dna marker bp 1 kb is designed for use as molecular weight standard for agarose gel electrophoresis features. How can i prepare working solution with 1 kb dna ladder supplied by promega. The ladder is composed of fifteen chromatographypurified individual dna. Dna ladder is commonly used to determine the size of dna fragments by electrophoresis in routine molecular biology laboratories. It allows sizing and concentration estimate of dna fragments on agarose gels generated by pcr or restriction digest. Pdf dna ladder is commonly used to determine the size of dna fragments by. Promega 1kb dna step ladder molecular weight marker for. The ppsu plasmids for generating dna molecular weight. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide.
This protocol is a combination of routinely employed methods. Norgen biotek was used as a size reference for the genomic dna samples. Sixteen dna fragments ranging from 50bp to 800bp in 50bp increments plus an 1,800bp backbone fragment. Often, dna molecular weight markers and ladders come in two formats. Please have a look into the related application note for more information about ladder migration in different gel concentrations. The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer.
Promega 1kb dna ladder molecular weight marker fisher scientific. Geneons 50 kb dna marker is composed of 17 individual dna fragments which are redissolved in storage buffer. Promega 1kb dna ladder molecular weight marker 1kb dna. The 300bp band is 3 times more intense than all other bands.
The digested dna includes fragments ranging from 0. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. Straightforward procedure for laboratory production of dna. This marker can be used with either agarose or polyacrylamide gels. The 1 kb and 3 kb bands contain more dna to provide internal orientation. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for.
These ladders are sometimes made from restriction enzyme digestion of plasmid dna with a known sequence. Find and purchase 1 kb plus dna ladder products at invitrogen life science technologies. In addition to these 12 bands, the ladder contains vector dna fragments that range from 75 to 1636 bp. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Promega 1kb dna step ladder molecular weight marker for electrophoresis ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. The product is supplied in a solution of 10 mm trishcl, ph 8.
The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for. The ladders are not intended for use in quantitative analysis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Dna ladders contain dna fragments of varying sizes. Comes supplied with 1 vial of gel loading dye, purple 6x, no sds. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. For na electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. The doublestranded dna ladder can be visualized on 12% agarose gels after ethidium bromide or sybr safe staining. Generuler 1 kb plus dna ladder thermo fisher scientific. The 500bp fragment is present at increased intensity for easy identification. Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments plus a band at 1,500bp. Identification of the substancemixture and of the companyundertaking product identifier 1 kb dna ladder or ruler trade namebrand invitrogen 1 kb plus dna ladder synonyms deoxyribonucleic acid, aqueous solution reach number mixture, not applicable. The 100 bp psti and 1 kb ecorv penn state dna ladders are shown together with the new england biolabs 2log dna ladder on a 1 % agarose gel. Thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp.
The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel loading dye, purple 6x, no sds. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. An excellent option to consider is the benchtop 1kb dna ladder. The ladder is composed of fourteen chromatographypurified. The 1kb dna step ladder has ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. Generuler 1 kb dna ladder 1250 25 x 50 g for 2500 applications, 0. Isolation of genomic dna from soil microorganisms without. View more information about our dna stains technology.
The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free. Pdf straightforward procedure for laboratory production of dna. The benchtop 1kb dna ladder has bluntended fragments with sizes ranging from 250bp to 10,000bp. Generuler 1 kb dna ladder thermo fisher scientific. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. The dna fragments may be stained with ethidium bromide. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and. Directload 1 kb dna ladder readytouse marker for dna.1645 189 674 954 1165 552 689 638 1621 1386 928 762 1092 1337 669 1059 1606 377 1667 232 1566 1632 749 1639 740 311 108 1607 1258 518 337 1328 1300 60 31 749